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Immunohistochemical staining of human lung shows moderate cytoplasmic and secreted positivity in macrophages.
Immunohistochemical staining of human lung shows moderate cytoplasmic and secreted positivity in macrophages.
Immunohistochemical staining of human lung shows moderate cytoplasmic and secreted positivity in macrophages.

Anti-XCL1 Antibody

HPA057725
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetXCL1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-XCL1
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6375
  • Target name
    XCL1
  • Target description
    X-C motif chemokine ligand 1
  • Target synonyms
    ATAC; c motif chemokine 1; chemokine (C motif) ligand 1; cytokine SCM-1; LPTN; LTN; lymphotactin; lymphotaxin; SCM1; SCM-1; SCM1A; SCM-1a; SCM-1-alpha; SCYC1; single cysteine motif 1a; small inducible cytokine subfamily C, member 1 (lymphotactin); small-inducible cytokine C1; XC chemokine ligand 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP47992
  • Protein Name
    Lymphotactin
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Lymphotactin using anti-Lymphotactin antibody (PB9681). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: HELA Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lymphotactin antigen affinity purified polyclonal antibody (Catalog # PB9681) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lymphotactin at approximately 13 kDa. The expected band size for Lymphotactin is at 13 kDa.
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