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Chemokine (C motif) ligand (XCL1) Polyclonal Antibody

CAU27122
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetXCL1
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Overview

  • Supplier
    Biomatik
  • Product Name
    Chemokine (C motif) ligand (XCL1) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    WB; IHC; ICC; IP.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6375
  • Target name
    XCL1
  • Target description
    X-C motif chemokine ligand 1
  • Target synonyms
    ATAC; c motif chemokine 1; chemokine (C motif) ligand 1; cytokine SCM-1; LPTN; LTN; lymphotactin; lymphotaxin; SCM1; SCM-1; SCM1A; SCM-1a; SCM-1-alpha; SCYC1; single cysteine motif 1a; small inducible cytokine subfamily C, member 1 (lymphotactin); small-inducible cytokine C1; XC chemokine ligand 1
  • Host
    Rabbit
  • Protein IDP47992
  • Protein Name
    Lymphotactin
  • Scientific Description
    The Chemokine (C motif) ligand (XCL1) Polyclonal Antibody (Species: Human) has been validated for the following applications: WB, IHC, ICC, IP.
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Lymphotactin using anti-Lymphotactin antibody (PB9681). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: HELA Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lymphotactin antigen affinity purified polyclonal antibody (Catalog # PB9681) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lymphotactin at approximately 13 kDa. The expected band size for Lymphotactin is at 13 kDa.
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