Anti-XCR1 [5G7]

Absolute Antibody

Anti-XCR1 [5G7]

9

This antibody binds human XCR1, more particularly it strongly binds amino acids PESTTFFYYDLQ of the human XCR1. XRC1 is a receptor for chemokines SCYC1 and SCYC2 and expressed on 80% of CD8+ the dendritic cells. It also acts as a receptor for XCL1/Lymphot

This antibody is capable of binding human XCR1-EGFP-expressing B300.19 cells in a flow cytometry analysis. The determination of the binding epitope of this antibody was done using ELISA (US9371389). This antibody exhibits a significant effect in treating rheumatoid arthritis in the experiment of delayed-type hypersensitivity reaction induced by Mycobacterium butyricum delayed-type hypersensitivity reaction. The neutralizing activity of this antibody against human lymphotactin-induced migration of human XCR1-expressing cells was evaluated by in vitro chemotaxis assay. It was reported that this antibody completely inhibited cell migration at a concentration of about 3 microg/mL (US9371389). The effect of this antibody on ear swelling of DNFB (Dinitrofluorobenzene)-sensitized mice was done and it was reported that this antibody caused significant suppression of ear swelling 24 after induction. This antibody was also reported to show showed a very low reactivity to human CX3CR1-EGFP-expressing B300.19 cells (US9371389). The original antibody with saporin-conjugated secondary antibody showed growth suppression of human XCR1-EGFP-expressing B300.19 cells. The humanized version of this antibody is also available on request (US9371389).

Flow Cytometry, ELISA

Research Use Only

Monoclonal

5G7

XCR1

X-C motif chemokine receptor 1

CCXCR1; chemokine (C motif) receptor 1; chemokine (C motif) XC receptor 1; chemokine XC receptor 1; G protein-coupled receptor 5; GPR5; lymphotactin receptor; XC chemokine receptor 1

Mouse

IgG2b

Chemokine XC receptor 1

Human

-20°C,2°C to 8°C

12352203