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Immunohistochemical staining of human endometrium shows moderate nuclear positivity in glandular cells.
Immunohistochemical staining of human endometrium shows moderate nuclear positivity in glandular cells.
Immunohistochemical staining of human endometrium shows moderate nuclear positivity in glandular cells.

Anti-YTHDC1 Antibody

HPA036462
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetYTHDC1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-YTHDC1 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID91746
  • Target name
    YTHDC1
  • Target description
    YTH N6-methyladenosine RNA binding protein C1
  • Target synonyms
    YT521, YT521-B, YTH domain-containing protein 1, YTH domain containing 1, putative splicing factor YT521, splicing factor YT521, splicing factor YT521-B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96MU7
  • Protein Name
    YTH domain-containing protein 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of YTHDC1 using anti-YTHDC1 antibody (A06172-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human U937 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human HEPG2 whole cell lysates, Lane 5: human HELA whole cell lysates, Lane 6: human Jurkat whole cell lysates, Lane 7: rat testis tissue lysates, Lane 8: rat C6 whole cell lysates, Lane 9: mouse testis tissue lysates, Lane 10: mouse RAW264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-YTHDC1 antigen affinity purified polyclonal antibody (Catalog # A06172-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for YTHDC1 at approximately 85KD. The expected band size for YTHDC1 is at 85KD.
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