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YTHDC1 Antibody

LS-C346402
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetYTHDC1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    YTHDC1 Antibody
  • Delivery Days Customer
    23
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IF (1:50 - 1:100), IHC (1:50 - 1:200), WB (1:500 - 1:2000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID91746
  • Target name
    YTHDC1
  • Target description
    YTH N6-methyladenosine RNA binding protein C1
  • Target synonyms
    YT521, YT521-B, YTH domain-containing protein 1, YTH domain containing 1, putative splicing factor YT521, splicing factor YT521, splicing factor YT521-B
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of YTHDC1 using anti-YTHDC1 antibody (A06172-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human U937 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human HEPG2 whole cell lysates, Lane 5: human HELA whole cell lysates, Lane 6: human Jurkat whole cell lysates, Lane 7: rat testis tissue lysates, Lane 8: rat C6 whole cell lysates, Lane 9: mouse testis tissue lysates, Lane 10: mouse RAW264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-YTHDC1 antigen affinity purified polyclonal antibody (Catalog # A06172-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for YTHDC1 at approximately 85KD. The expected band size for YTHDC1 is at 85KD.
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