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WB analysis of various sample lysates using GTX32976 YTHDC1 antibody. The signal was developed with ECL plus-Enhanced. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX32976 YTHDC1 antibody. The signal was developed with ECL plus-Enhanced. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX32976 YTHDC1 antibody. The signal was developed with ECL plus-Enhanced. Dilution : 1:1000 Loading : 25microg per lane

YTHDC1 antibody

GTX32976
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetYTHDC1
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Overview

  • Supplier
    GeneTex
  • Product Name
    YTHDC1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID91746
  • Target name
    YTHDC1
  • Target description
    YTH N6-methyladenosine RNA binding protein C1
  • Target synonyms
    YT521, YT521-B, YTH domain-containing protein 1, YTH domain containing 1, putative splicing factor YT521, splicing factor YT521, splicing factor YT521-B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96MU7
  • Protein Name
    YTH domain-containing protein 1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Li N, Zhu Z, Deng Y, et al. KIAA1429/VIRMA promotes breast cancer progression by m(6) A-dependent cytosolic HAS2 stabilization. EMBO Rep. 2023,24(10):e55506. doi: 10.15252/embr.202255506
    Read this paper
  • Zhu Y, Wang R, Zou J, et al. N6-methyladenosine reader protein YTHDC1 regulates influenza A virus NS segment splicing and replication. PLoS Pathog. 2023,19(4):e1011305. doi: 10.1371/journal.ppat.1011305
    Read this paper
  • He JJ, Li Z, Rong ZX, et al. m(6)A Reader YTHDC2 Promotes Radiotherapy Resistance of Nasopharyngeal Carcinoma via Activating IGF1R/AKT/S6 Signaling Axis. Front Oncol. 2020,10:1166. doi: 10.3389/fonc.2020.01166
    Read this paper

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Figure 1. Western blot analysis of YTHDC1 using anti-YTHDC1 antibody (A06172-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human U937 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human HEPG2 whole cell lysates, Lane 5: human HELA whole cell lysates, Lane 6: human Jurkat whole cell lysates, Lane 7: rat testis tissue lysates, Lane 8: rat C6 whole cell lysates, Lane 9: mouse testis tissue lysates, Lane 10: mouse RAW264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-YTHDC1 antigen affinity purified polyclonal antibody (Catalog # A06172-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for YTHDC1 at approximately 85KD. The expected band size for YTHDC1 is at 85KD.
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