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Immunohistochemistry analysis in human tonsil and kidney tissues using Anti-ZBP1 antibody. Corresponding ZBP1 RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human tonsil and kidney tissues using Anti-ZBP1 antibody. Corresponding ZBP1 RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human tonsil and kidney tissues using Anti-ZBP1 antibody. Corresponding ZBP1 RNA-seq data are presented for the same tissues.

Anti-ZBP1 Antibody

HPA041256
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetZBP1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ZBP1
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID81030
  • Target name
    ZBP1
  • Target description
    Z-DNA binding protein 1
  • Target synonyms
    C20orf183, DAI, DLM-1, DLM1, Z-DNA-binding protein 1, DNA-dependent activator of IRFs, tumor stroma and activated macrophage protein DLM-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H171
  • Protein Name
    Z-DNA-binding protein 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of DAI/ZBP1 using anti-DAI/ZBP1 antibody (A04739-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DAI/ZBP1 antigen affinity purified polyclonal antibody (Catalog # A04739-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DAI/ZBP1 at approximately 63KD. The expected band size for DAI/ZBP1 is at 63KD.
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