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Western Blot analysis of mouse ZBP1 in L929 cells by using anti-ZBP1, mAb (Zippy-1) (Prod. No. AG-20B-0010). Cell extracts from L929 cell either unstimulated (lane 1) or stimulated for 24h with poly(dA.dT) poly(dT.dA) at 3microg/ml (lane 2) were reso
Western Blot analysis of mouse ZBP1 in L929 cells by using anti-ZBP1, mAb (Zippy-1) (Prod. No. AG-20B-0010). Cell extracts from L929 cell either unstimulated (lane 1) or stimulated for 24h with poly(dA.dT) poly(dT.dA) at 3microg/ml (lane 2) were reso
Western Blot analysis of mouse ZBP1 in L929 cells by using anti-ZBP1, mAb (Zippy-1) (Prod. No. AG-20B-0010). Cell extracts from L929 cell either unstimulated (lane 1) or stimulated for 24h with poly(dA.dT) poly(dT.dA) at 3microg/ml (lane 2) were reso

anti-ZBP1, mAb (Zippy-1)

AG-20B-0010
AdipoGen Life Sciences
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetZBP1
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Overview

  • Supplier
    AdipoGen Life Sciences
  • Product Name
    anti-ZBP1, mAb (Zippy-1)
  • Delivery Days Customer
    10
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    Zippy-1
  • Concentration
    1 mg/ml
  • Estimated Purity
    >95%
  • Gene ID81030
  • Target name
    ZBP1
  • Target description
    Z-DNA binding protein 1
  • Target synonyms
    C20orf183; DAI; DLM1; DLM-1; DNA-dependent activator of IRFs; tumor stroma and activated macrophage protein DLM-1; Z-DNA-binding protein 1
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDQ9H171
  • Protein Name
    Z-DNA-binding protein 1
  • Scientific Description
    Monoclonal Antibody. Recognizes human and mouse ZBP1. Isotype: Mouse IgG2a. Clone: Zippy-1. Applications: ICC, IP, WB. Liquid. In PBS containing 10% glycerol and 0.02% sodium azide. ZBP1 is an interferon inducible protein that binds to cytoplasmic DNA and plays a role in host defense against tumors and pathogens. ZBP1 is highly expressed in lymphatic tissues including lymph node, leukocytes, tonsil, bone marrow and spleen. ZBP1 is an innate immune sensor of Influenza A virus (IAV). ZBP1 regulates NLRP3 (nucleotide and oligomerization domain, leucine-rich repeat-containing protein family, pyrin domain containing 3) inflammasome activation as well as induction of different types of cell death (apoptosis, necroptosis and pyroptosis) during IAV infection. - ZBP1 is an interferon inducible protein that binds to cytoplasmic DNA and plays a role in host defense against tumors and pathogens. ZBP1 is highly expressed in lymphatic tissues including lymph node, leukocytes, tonsil, bone marrow and spleen. ZBP1 is an innate immune sensor of Influenza A virus (IAV). ZBP1 regulates NLRP3 (nucleotide and oligomerization domain, leucine-rich repeat-containing protein family, pyrin domain containing 3) inflammasome activation as well as induction of different types of cell death (apoptosis, necroptosis and pyroptosis) during IAV infection.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DAI/ZBP1 using anti-DAI/ZBP1 antibody (A04739-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DAI/ZBP1 antigen affinity purified polyclonal antibody (Catalog # A04739-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DAI/ZBP1 at approximately 63KD. The expected band size for DAI/ZBP1 is at 63KD.
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