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ProductsBack/ZBP1 antibody, Internal
WB analysis of Jurkat cells using GTX47462 ZBP1 antibody at 5.0microg/ml.
WB analysis of Jurkat cells using GTX47462 ZBP1 antibody at 5.0microg/ml.
WB analysis of Jurkat cells using GTX47462 ZBP1 antibody at 5.0microg/ml.

ZBP1 antibody, Internal

GTX47462
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetZBP1
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Overview

  • Supplier
    GeneTex
  • Product Name
    ZBP1 antibody, Internal
  • Delivery Days Customer
    9
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5-1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID81030
  • Target name
    ZBP1
  • Target description
    Z-DNA binding protein 1
  • Target synonyms
    C20orf183; DAI; DLM1; DLM-1; DNA-dependent activator of IRFs; tumor stroma and activated macrophage protein DLM-1; Z-DNA-binding protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H171
  • Protein Name
    Z-DNA-binding protein 1
  • Scientific Description
    This gene encodes a Z-DNA binding protein. The encoded protein plays a role in the innate immune response by binding to foreign DNA and inducing type-I interferon production. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DAI/ZBP1 using anti-DAI/ZBP1 antibody (A04739-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DAI/ZBP1 antigen affinity purified polyclonal antibody (Catalog # A04739-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DAI/ZBP1 at approximately 63KD. The expected band size for DAI/ZBP1 is at 63KD.
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