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Immunohistochemical staining of human Testis shows moderate nuclear positivity in spermatogonia.
Immunohistochemical staining of human Testis shows moderate nuclear positivity in spermatogonia.
Immunohistochemical staining of human Testis shows moderate nuclear positivity in spermatogonia.

Anti-ZBTB16 Antibody

HPA001499
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetZBTB16
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ZBTB16
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7704
  • Target name
    ZBTB16
  • Target description
    zinc finger and BTB domain containing 16
  • Target synonyms
    PLZF, ZNF145, zinc finger and BTB domain-containing protein 16, promyelocytic leukaemia zinc finger, zinc finger protein 145 (Kruppel-like, expressed in promyelocytic leukemia), zinc finger protein PLZF
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ05516
  • Protein Name
    Zinc finger and BTB domain-containing protein 16
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of Plzf/ZBTB1 using Plzf/ZBTB1 antibody (A00817-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: human Raji whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: rat spleen tissue lysates, Lane 8: mouse Raw264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Plzf/ZBTB1 antigen affinity purified polyclonal antibody (Catalog # A00817-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Plzf/ZBTB1 at approximately 74KD. The expected band size for Plzf/ZBTB1 is at 74KD.
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