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ProductsBack/Anti-ZBTB16 [RAB-C208]

Anti-ZBTB16 [RAB-C208]

Ab01928-1.1
Absolute Antibody
ApplicationsFlow Cytometry, ImmunoFluorescence
Product group Antibodies
TargetZBTB16
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-ZBTB16 [RAB-C208]
  • Delivery Days Customer
    7
  • Application Supplier Note
    This antibody has been used and recommended for the following methods: FC; spiked-IP; IF.
  • Applications
    Flow Cytometry, ImmunoFluorescence
  • Applications Supplier
    FC; spiked-IP; IF
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    RAB-C208
  • Gene ID7704
  • Target name
    ZBTB16
  • Target description
    zinc finger and BTB domain containing 16
  • Target synonyms
    PLZF; promyelocytic leukaemia zinc finger; zinc finger and BTB domain-containing protein 16; zinc finger protein 145 (Kruppel-like, expressed in promyelocytic leukemia); zinc finger protein PLZF; ZNF145
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ05516
  • Protein Name
    Zinc finger and BTB domain-containing protein 16
  • Scientific Description
    This chimeric mouse antibody was made using the variable domain sequences of the original Human Fab format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    This antibody was obtained by recombinant antibody (rAb) phage display recognizing ZBTB16 protein under non-denaturing conditions.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Plzf/ZBTB1 using Plzf/ZBTB1 antibody (A00817-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: human Raji whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: rat spleen tissue lysates, Lane 8: mouse Raw264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Plzf/ZBTB1 antigen affinity purified polyclonal antibody (Catalog # A00817-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Plzf/ZBTB1 at approximately 74KD. The expected band size for Plzf/ZBTB1 is at 74KD.
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