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ZBTB16 Polyclonal Antibody

CAC12930
Biomatik
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityMouse
TargetZBTB16
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Overview

  • Supplier
    Biomatik
  • Product Name
    ZBTB16 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7704
  • Target name
    ZBTB16
  • Target description
    zinc finger and BTB domain containing 16
  • Target synonyms
    PLZF, ZNF145, zinc finger and BTB domain-containing protein 16, promyelocytic leukaemia zinc finger, zinc finger protein 145 (Kruppel-like, expressed in promyelocytic leukemia), zinc finger protein PLZF
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ05516
  • Protein Name
    Zinc finger and BTB domain-containing protein 16
  • Scientific Description
    The ZBTB16 Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IHC.
  • Reactivity
    Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Plzf/ZBTB1 using Plzf/ZBTB1 antibody (A00817-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: human Raji whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: rat spleen tissue lysates, Lane 8: mouse Raw264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Plzf/ZBTB1 antigen affinity purified polyclonal antibody (Catalog # A00817-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Plzf/ZBTB1 at approximately 74KD. The expected band size for Plzf/ZBTB1 is at 74KD.
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PLZF antibody [N1N3] detects PLZF protein at nucleus by immunofluorescent analysis. Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PLZF protein stained by PLZF antibody [N1N3] (GTX111046) diluted at 1:2000. Blue: Hoechst 33342 staining.
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