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Immunohistochemical staining of human adrenal gland shows strong nuclear and cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human adrenal gland shows strong nuclear and cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human adrenal gland shows strong nuclear and cytoplasmic positivity in glandular cells.

Anti-ZNF318 Antibody

HPA027031
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetZNF318
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ZNF318
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID24149
  • Target name
    ZNF318
  • Target description
    zinc finger protein 318
  • Target synonyms
    HRIHFB2436, TZF, ZFP318, zinc finger protein 318, endocrine regulator, endocrine regulatory protein, testicular zinc finger
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ5VUA4
  • Protein Name
    Zinc finger protein 318
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ZNF318 using anti-ZNF318 antibody (A11267-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: rat stomach tissue lysates, Lane 3: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZNF318 antigen affinity purified polyclonal antibody (Catalog # A11267-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZNF318 at approximately 290 kDa. The expected band size for ZNF318 is at 251 kDa.
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Lane 1: Mouse Lymph node lysates; Lane 2: Mouse Testis lysates; Lane 3: Human A549 cell lysates; Lane 4: Human HL-60 cell lysates; Lane 5: Human 293T cell lysates probed with ZNF318 Polyclonal Antibody, Unconjugated (bs-11414R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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