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Antibodies

We offer one of the most comprehensive portfolios of antibodies. This includes monoclonal and polyclonal primary, secondary, conjugated, phospho-specific, functional, (isotype) controls, tagged and antibody pairs. In addition, we offer custom antibody services from several manufacturers.

The antibodies are generated in various hosts and react to antigens of different species like human, mouse, rat, rabbit or zebrafish. The antibodies are validated for multiple applications, including immunohistochemistry, western blot, immunoprecipitation, ELISA and flow cytometry, to ensure reliable performance for your research needs.

If you need help finding the right antibodies for your research, please use our antibody request form.

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IHC analysis of GZMB using anti-GZMB antibody (M00353-1) on paraffin-embedded human T cell lymphoma. GZMB was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GZMB Antibody (M00353-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
ApplicationsImmunoFluorescence, ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetGZMB
Western blot analysis of CEA(CD66e) expression in Human colon cancer lysate (M00356-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEACAM5 monoclonal antibody (Catalog # M00356-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Product group Antibodies
Boster Bio
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetCEACAM5
Immunohistochemical analysis of paraffin-embedded human spleen, using CD11c Antibody.
Product group Antibodies
Boster Bio
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetITGAX
Western blot analysis of CDK6 expression in HeLa cell lysate (M00358). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK6 monoclonal antibody (Catalog # M00358) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDK6
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetCDK6
Western blot analysis of TG using anti-TG antibody (M00359-3) in Mouse thyroid lysate. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TG antigen affinity purified polyclonal antibody (Catalog # M00359-3) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetTG
Western blot analysis of TG expression in Human thyroid lysate using anti-TG antibody (M00359-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TG antigen affinity purified polyclonal antibody (Catalog # M00359-4) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for TG.
Product group Antibodies
Boster Bio
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetTG
Western blot analysis of extracts of NIH/3T3 cell lysate, using CD47 antibody.
Product group Antibodies
References
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetCD47
Western blot analysis of TMS1 expression in U937 cell lysate (M00362). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PYCARD monoclonal antibody (Catalog # M00362) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PYCARD
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman
TargetPYCARD
IHC analysis of Elafin using anti-Elafin antibody (M00364). Elafin was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Elafin Antibody (M00364) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
Product group Antibodies
Boster Bio
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetPI3
Western blot analysis of PDGFR expression in SH-SY5Y cell lysate.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetPDGFRA
Western blot analysis of iNOS expression in Human fetal brain lysate.
Product group Antibodies
Boster Bio
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse
TargetNOS2
Western blot analysis of ATF4 expression in (1) HeLa cell lysate; (2) 3T3 cell lysate.
Product group Antibodies
References
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetATF4