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ProductsBack/Antithrombin III antibody [8D5]
FACS analysis of HEK293T cells transfected with either Antithrombin III plasmid(Red) or empty vector control plasmid(Blue) using GTX83659 Antithrombin III antibody [8D5].
FACS analysis of HEK293T cells transfected with either Antithrombin III plasmid(Red) or empty vector control plasmid(Blue) using GTX83659 Antithrombin III antibody [8D5].
FACS analysis of HEK293T cells transfected with either Antithrombin III plasmid(Red) or empty vector control plasmid(Blue) using GTX83659 Antithrombin III antibody [8D5].

Antithrombin III antibody [8D5]

GTX83659
GeneTex
ApplicationsFlow Cytometry, Western Blot
DownloadDatasheet
Product group Antibodies
TargetSERPINC1
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Overview

  • Supplier
    GeneTex
  • Product Name
    Antithrombin III antibody [8D5]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    8D5
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID462
  • Target name
    SERPINC1
  • Target description
    serpin family C member 1
  • Target synonyms
    antithrombin-III; AT3; AT3D; ATIII; ATIII-R2; ATIII-T1; ATIII-T2; serine (or cysteine) proteinase inhibitor, clade C (antithrombin), member 1; serpin peptidase inhibitor clade C member 1; serpin peptidase inhibitor, clade C (antithrombin), member 1; THPH7
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDP01008
  • Protein Name
    Antithrombin-III
  • Scientific Description
    Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Antithrombin III/SERPINC1 using anti-Antithrombin III/SERPINC1 antibody (A00469-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse testis tissue lysates, Lane 3: mouse ovary tissue lysates, Lane 4: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Antithrombin III/SERPINC1 antigen affinity purified polyclonal antibody (Catalog # A00469-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Antithrombin III/SERPINC1 at approximately 53 kDa. The expected band size for Antithrombin III/SERPINC1 is at 52 kDa.
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