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ProductsBack/ANXA2 / Annexin A2 Antibody

ANXA2 / Annexin A2 Antibody

LS-C400458
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetANXA2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ANXA2 / Annexin A2 Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, IHC (1:25 - 1:100), WB (1:1000 - 1:5000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.4 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID302
  • Target name
    ANXA2
  • Target description
    annexin A2
  • Target synonyms
    ANX2, ANX2L4, CAL1H, HEL-S-270, LIP2, LPC2, LPC2D, P36, PAP-IV, annexin A2, annexin II, annexin-2, calpactin I heavy chain, calpactin I heavy polypeptide, calpactin-1 heavy chain, chromobindin 8, epididymis secretory protein Li 270, epididymis secretory sperm binding protein, lipocortin II, placental anticoagulant protein IV, protein I
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Annexin A2/ANXA2 using anti-Annexin A2/ANXA2 antibody (A00868-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human placenta tissue lysates, Lane 5: rat kidney tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse kidney tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Annexin A2/ANXA2 antigen affinity purified polyclonal antibody (Catalog # A00868-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Annexin A2/ANXA2 at approximately 36 kDa. The expected band size for Annexin A2/ANXA2 is at 39 kDa.
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