IHC-P analysis of human breast carcinoma tissue using GTX15878 AP2 alpha antibody [3B5]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:50
![WB analysis of whole cell extracts (30 microg lysate) of PC-3 (Lane1), A-431 (Lane2), Mouse Placenta (Lane3) and RAW 264.7 (Lane5) using GTX15878 AP2 alpha antibody [3B5]. Dilution : 1:50-1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX15878/GTX15878_1549_WB_w_23060620_866.webp "WB analysis of whole cell extracts (30 microg lysate) of PC-3 (Lane1), A-431 (Lane2), Mouse Placenta (Lane3) and RAW 264.7 (Lane5) using GTX15878 AP2 alpha antibody [3B5]. Dilution : 1:50-1:200")
![ICC/IF analysis of MCF-7 cells using GTX15878 AP2 alpha antibody [3B5]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX15878/GTX15878_343_ICC-IF_w_23060620_416.webp "ICC/IF analysis of MCF-7 cells using GTX15878 AP2 alpha antibody [3B5]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC")
![ICC/IF analysis of HeLa cells using GTX15878 AP2 alpha antibody [3B5]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX15878/GTX15878_344_ICC-IF_w_23060620_828.webp "ICC/IF analysis of HeLa cells using GTX15878 AP2 alpha antibody [3B5]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC")
![ICC/IF analysis of U251 cells using GTX15878 AP2 alpha antibody [3B5]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX15878/GTX15878_345_ICC-IF_w_23060620_579.webp "ICC/IF analysis of U251 cells using GTX15878 AP2 alpha antibody [3B5]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC")
IHC-P analysis of human breast carcinoma tissue using GTX15878 AP2 alpha antibody [3B5]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:50
AP2 alpha antibody [3B5]
GTX15878
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityChicken, Human, Mouse
TargetTFAP2A
Overview
- SupplierGeneTex
- Product NameAP2 alpha antibody [3B5]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:50 - 1:200. ICC/IF: 1:50-1:500. IHC-P: 1:50. IP: 1:100 - 1:250. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID3B5
- ConjugateUnconjugated
- Gene ID7020
- Target nameTFAP2A
- Target descriptiontranscription factor AP-2 alpha
- Target synonymsactivating enhancer-binding protein 2-alpha; activator protein 2; AP-2; AP-2 transcription factor; AP-2alpha; AP2TF; BOFS; TFAP2; transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha); transcription factor AP-2-alpha
- HostMouse
- IsotypeIgG2b
- Protein IDP05549
- Protein NameTranscription factor AP-2-alpha
- Scientific DescriptionThe protein encoded by this gene is a transcription factor that binds the consensus sequence 5-GCCNNNGGC-3. The encoded protein functions as either a homodimer or as a heterodimer with similar family members. This protein activates the transcription of some genes while inhibiting the transcription of others. Defects in this gene are a cause of branchiooculofacial syndrome (BOFS). Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Dec 2009]
- ReactivityChicken, Human, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203