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ProductsBack/AP2 alpha antibody [3B5]
IHC-P analysis of human breast carcinoma tissue using GTX15878 AP2 alpha antibody [3B5]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:50
IHC-P analysis of human breast carcinoma tissue using GTX15878 AP2 alpha antibody [3B5]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:50
IHC-P analysis of human breast carcinoma tissue using GTX15878 AP2 alpha antibody [3B5]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:50

AP2 alpha antibody [3B5]

GTX15878
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetTFAP2A
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Overview

  • Supplier
    GeneTex
  • Product Name
    AP2 alpha antibody [3B5]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:50 - 1:200. ICC/IF: 1:50-1:500. IHC-P: 1:50. IP: 1:100 - 1:250. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    3B5
  • Conjugate
    Unconjugated
  • Gene ID7020
  • Target name
    TFAP2A
  • Target description
    transcription factor AP-2 alpha
  • Target synonyms
    AP-2, AP-2alpha, AP2TF, BOFS, TFAP2, transcription factor AP-2-alpha, AP-2 transcription factor, activating enhancer-binding protein 2-alpha, activator protein 2, transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha)
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDP05549
  • Protein Name
    Transcription factor AP-2-alpha
  • Scientific Description
    The protein encoded by this gene is a transcription factor that binds the consensus sequence 5-GCCNNNGGC-3. The encoded protein functions as either a homodimer or as a heterodimer with similar family members. This protein activates the transcription of some genes while inhibiting the transcription of others. Defects in this gene are a cause of branchiooculofacial syndrome (BOFS). Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Dec 2009]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of AP2 alpha using anti-AP2 alpha antibody (PB9118). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human AP2A protein 0.5 ng. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AP2 alpha antigen affinity purified polyclonal antibody (Catalog # PB9118) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AP2 alpha at approximately 38 kDa. The expected band size for AP2 alpha is at 38 kDa.
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