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Western Blot analysis of COLO205 cells using AP-2alpha/beta Polyclonal Antibody
Western Blot analysis of COLO205 cells using AP-2alpha/beta Polyclonal Antibody
Western Blot analysis of COLO205 cells using AP-2alpha/beta Polyclonal Antibody

TFAP2A/TFAP2B Antibody

CSB-PA000893
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTFAP2A
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Overview

  • Supplier
    Cusabio
  • Product Name
    TFAP2A/TFAP2B Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7020
  • Target name
    TFAP2A
  • Target description
    transcription factor AP-2 alpha
  • Target synonyms
    AP-2, AP-2alpha, AP2TF, BOFS, TFAP2, transcription factor AP-2-alpha, AP-2 transcription factor, activating enhancer-binding protein 2-alpha, activator protein 2, transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP05549
  • Protein Name
    Transcription factor AP-2-alpha
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of AP2 alpha using anti-AP2 alpha antibody (PB9118). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human AP2A protein 0.5 ng. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AP2 alpha antigen affinity purified polyclonal antibody (Catalog # PB9118) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AP2 alpha at approximately 38 kDa. The expected band size for AP2 alpha is at 38 kDa.
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