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AP2M1 antibody

10R-3324
Biosynth
Product group Antibodies
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Overview

  • Supplier
    Biosynth
  • Product Name
    AP2M1 antibody
  • Delivery Days Customer
    11
  • Certification
    Research Use Only
  • UNSPSC
    41116161

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Western blot All lanes: AP2M1 antibody at 2microg/ml Lane 1: Jurket whole cell lysate Lane 2: U87 whole cell lysate Lane 3: U251 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 50 kDa Observed band size: 50, 22 kDa
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Figure 1. Western blot analysis of AP2M1 using anti-AP2M1 antibody (A06179-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AP2M1 antigen affinity purified polyclonal antibody (Catalog # A06179-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AP2M1 at approximately 50 kDa. The expected band size for AP2M1 is at 50 kDa.
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Sample(30 μg of whole cell lysate) A:293T B:HeLa S3(GTX14654) 10% SDS PAGE GTX101502 diluted at 1:500
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