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AP50 / AP2M1 Antibody

LS-C748977
LifeSpan BioSciences
ApplicationsImmunoFluorescence
Product group Antibodies
TargetAP2M1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    AP50 / AP2M1 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 49kDa.
  • Applications
    ImmunoFluorescence
  • Applications Supplier
    IF (1:50 - 1:100) The predicted MW is 49kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID1173
  • Target name
    AP2M1
  • Target description
    adaptor related protein complex 2 subunit mu 1
  • Target synonyms
    AP50, CLAPM1, MRD60, mu2, AP-2 complex subunit mu, AP-2 mu 2 chain, HA2 50 kDA subunit, adaptin-mu2, adaptor protein complex AP-2 subunit mu, adaptor related protein complex 2 mu 1 subunit, adaptor-related protein complex 2 subunit mu, clathrin adaptor complex AP2, mu subunit, clathrin assembly protein complex 2 medium chain, clathrin assembly protein complex 2 mu medium chain, clathrin coat adaptor protein AP50, clathrin coat assembly protein AP50, clathrin coat-associated protein AP50, clathrin-associated/assembly/adaptor protein, medium 1, plasma membrane adaptor AP-2 50 kDa protein, plasma membrane adaptor AP-2 50kDA protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of AP2M1 using anti-AP2M1 antibody (A06179-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AP2M1 antigen affinity purified polyclonal antibody (Catalog # A06179-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AP2M1 at approximately 50 kDa. The expected band size for AP2M1 is at 50 kDa.
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