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ProductsBack/APAF1 Antibody - middle region (OASG00437)

APAF1 Antibody - middle region (OASG00437)

OASG00437
Aviva Systems Biology
ApplicationsWestern Blot
Product group Antibodies
TargetAPAF1
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    APAF1 Antibody - middle region (OASG00437)
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Format
    Liquid. 1 mg/mL in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • Gene ID317
  • Target name
    APAF1
  • Target description
    apoptotic peptidase activating factor 1
  • Target synonyms
    APAF-1, CED4, apoptotic protease-activating factor 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of APAF1 using anti-APAF1 antibody (A00889-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APAF1 antigen affinity purified polyclonal antibody (Catalog # A00889-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APAF1 at approximately 135 kDa. The expected band size for APAF1 is at 135 kDa.
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