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Western Blot analysis of A549 cells using RIAM Polyclonal Antibody
Western Blot analysis of A549 cells using RIAM Polyclonal Antibody
Western Blot analysis of A549 cells using RIAM Polyclonal Antibody

APBB1IP Antibody

CSB-PA030130
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetAPBB1IP
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Overview

  • Supplier
    Cusabio
  • Product Name
    APBB1IP Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID54518
  • Target name
    APBB1IP
  • Target description
    amyloid beta precursor protein binding family B member 1 interacting protein
  • Target synonyms
    INAG1, PREL1, RARP1, RIAM, amyloid beta A4 precursor protein-binding family B member 1-interacting protein, APBB1-interacting protein 1, MLLT10/APBB1IP reciprocal fusion, MLLT10/APBB1IP reciprocal fusion protein, PREL-1, RARP-1, Rap1-GTP-interacting adaptor molecule, Rap1-interacting adaptor molecule, proline rich EVH1 ligand 1, proline-rich protein 73, rap1-GTP-interacting adapter molecule, retinoic acid-responsive proline-rich protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ7Z5R6
  • Protein Name
    Amyloid beta A4 precursor protein-binding family B member 1-interacting protein
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of APBB1IP using anti-APBB1IP antibody (PA1960 ). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: Jurkat whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: U937 whole cell lysates, Lane 5: rat thymus tissue lysates, Lane 6: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APBB1IP antigen affinity purified polyclonal antibody (Catalog # PA1960 ) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APBB1IP at approximately 110-120KD. The expected band size for APBB1IP is at 73KD.
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