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ProductsBack/APBB1IP / RIAM Antibody (aa1-150)

APBB1IP / RIAM Antibody (aa1-150)

LS-C377995
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Rat
TargetAPBB1IP
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    APBB1IP / RIAM Antibody (aa1-150)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA, IHC, IHC-P (1:20 - 1:200), WB (1:200 - 1:1000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID54518
  • Target name
    APBB1IP
  • Target description
    amyloid beta precursor protein binding family B member 1 interacting protein
  • Target synonyms
    INAG1, PREL1, RARP1, RIAM, amyloid beta A4 precursor protein-binding family B member 1-interacting protein, APBB1-interacting protein 1, MLLT10/APBB1IP reciprocal fusion, MLLT10/APBB1IP reciprocal fusion protein, PREL-1, RARP-1, Rap1-GTP-interacting adaptor molecule, Rap1-interacting adaptor molecule, proline rich EVH1 ligand 1, proline-rich protein 73, rap1-GTP-interacting adapter molecule, retinoic acid-responsive proline-rich protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C,-80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of APBB1IP using anti-APBB1IP antibody (PA1960 ). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: Jurkat whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: U937 whole cell lysates, Lane 5: rat thymus tissue lysates, Lane 6: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APBB1IP antigen affinity purified polyclonal antibody (Catalog # PA1960 ) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APBB1IP at approximately 110-120KD. The expected band size for APBB1IP is at 73KD.
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