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ProductsBack/RIAM antibody [EPR2806]
Western blot analysis on (A) Human Lymph node (B) Fetal brain (C) human spleen (D) Jurkat and (E) HL-60 + retinoic acid cell lysates using anti-RIAM RabMAb (cat. #GTX62255).
Western blot analysis on (A) Human Lymph node (B) Fetal brain (C) human spleen (D) Jurkat and (E) HL-60 + retinoic acid cell lysates using anti-RIAM RabMAb (cat. #GTX62255).
Western blot analysis on (A) Human Lymph node (B) Fetal brain (C) human spleen (D) Jurkat and (E) HL-60 + retinoic acid cell lysates using anti-RIAM RabMAb (cat. #GTX62255).

RIAM antibody [EPR2806]

GTX62255
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetAPBB1IP
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Overview

  • Supplier
    GeneTex
  • Product Name
    RIAM antibody [EPR2806]
  • Delivery Days Customer
    9
  • Application Supplier Note
    Recommended Starting Dilutions:For WB: Use at a dilution of 1:2000 - 10,000For IHC: Use at a dilution of 1:100 - 250For ICC: Use at a dilution of 1:100 - 250For IP: Use at a dilution of 1:50Optimal working dilution for a specific application should be determined by the investigator.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    EPR2806
  • Conjugate
    Unconjugated
  • Gene ID54518
  • Target name
    APBB1IP
  • Target description
    amyloid beta precursor protein binding family B member 1 interacting protein
  • Target synonyms
    INAG1, PREL1, RARP1, RIAM, amyloid beta A4 precursor protein-binding family B member 1-interacting protein, APBB1-interacting protein 1, MLLT10/APBB1IP reciprocal fusion, MLLT10/APBB1IP reciprocal fusion protein, PREL-1, RARP-1, Rap1-GTP-interacting adaptor molecule, Rap1-interacting adaptor molecule, proline rich EVH1 ligand 1, proline-rich protein 73, rap1-GTP-interacting adapter molecule, retinoic acid-responsive proline-rich protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ7Z5R6
  • Protein Name
    Amyloid beta A4 precursor protein-binding family B member 1-interacting protein
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of APBB1IP using anti-APBB1IP antibody (PA1960 ). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: Jurkat whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: U937 whole cell lysates, Lane 5: rat thymus tissue lysates, Lane 6: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APBB1IP antigen affinity purified polyclonal antibody (Catalog # PA1960 ) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APBB1IP at approximately 110-120KD. The expected band size for APBB1IP is at 73KD.
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