IHC-P analysis of human liver tissue sections using GTX15658 Apolipoprotein A1 antibody [513]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : microwaved for 10 minutes in 10mM sodium citrate, pH 6.0 Dilution : 1:50
![WB analysis of various samples using GTX15658 Apolipoprotein A1 antibody [513]. Left Lane : 10ul of serum depleted of highly abundant proteins using the Top 2 Abundant Protein Depletion Spin Columns, and concentrated using the PES Protein Concentrators, 10K MWCO. Middle Lane : 10ul of serum depleted of highly abundant proteins using the Top 12 Abundant Protein Depletion Spin Columns, and concentrated using the PES Protein Concentrators, 10K MWCO. Right Lane : 50 ug of HepG2 whole cell lysate Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX15658/GTX15658_1505_WB_w_23060620_518.webp "WB analysis of various samples using GTX15658 Apolipoprotein A1 antibody [513]. Left Lane : 10ul of serum depleted of highly abundant proteins using the Top 2 Abundant Protein Depletion Spin Columns, and concentrated using the PES Protein Concentrators, 10K MWCO. Middle Lane : 10ul of serum depleted of highly abundant proteins using the Top 12 Abundant Protein Depletion Spin Columns, and concentrated using the PES Protein Concentrators, 10K MWCO. Right Lane : 50 ug of HepG2 whole cell lysate Dilution : 1:1000")
![WB analysis of the indicated amounts of a recombinant human Apo A-1-hIgG1Fc fusion protein or a recombinant mouse Apo A-1-hIgG1Fc fusion protein using GTX15658 Apolipoprotein A1 antibody [513]. Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX15658/GTX15658_1506_WB_w_23060620_592.webp "WB analysis of the indicated amounts of a recombinant human Apo A-1-hIgG1Fc fusion protein or a recombinant mouse Apo A-1-hIgG1Fc fusion protein using GTX15658 Apolipoprotein A1 antibody [513]. Dilution : 1:1000")
![Sandwich ELISA analysis of recombinant human Apo A-1 standards ranging from 1.6-1000ng/ml (A) or 100ul of diluted normal human serum or diluted serum from a patient with dyslipidemia (B) using GTX15658 Apolipoprotein A1 antibody [513] (detection antibody) and Apo A-1 rabbit monoclonal antibody (capture antibody).](https://www.genetex.com/upload/website/prouct_img/normal/GTX15658/GTX15658_29_ELISA_w_23060620_215.webp "Sandwich ELISA analysis of recombinant human Apo A-1 standards ranging from 1.6-1000ng/ml (A) or 100ul of diluted normal human serum or diluted serum from a patient with dyslipidemia (B) using GTX15658 Apolipoprotein A1 antibody [513] (detection antibody) and Apo A-1 rabbit monoclonal antibody (capture antibody).")
![Sandwich ELISA analysis of recombinant human Apo A-1 or recombinant mouse Apo A-1 protein ranging from 1.6-1000ng/ml using GTX15658 Apolipoprotein A1 antibody [513] (detection antibody) and Apo A-1 rabbit monoclonal antibody (capture antibody).](https://www.genetex.com/upload/website/prouct_img/normal/GTX15658/GTX15658_30_ELISA_w_23060620_786.webp "Sandwich ELISA analysis of recombinant human Apo A-1 or recombinant mouse Apo A-1 protein ranging from 1.6-1000ng/ml using GTX15658 Apolipoprotein A1 antibody [513] (detection antibody) and Apo A-1 rabbit monoclonal antibody (capture antibody).")
IHC-P analysis of human liver tissue sections using GTX15658 Apolipoprotein A1 antibody [513]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : microwaved for 10 minutes in 10mM sodium citrate, pH 6.0 Dilution : 1:50
Apolipoprotein A1 antibody [513]
GTX15658
ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, RadioImmunoAssay
Product group Antibodies
ReactivityHuman
TargetAPOA1
Overview
- SupplierGeneTex
- Product NameApolipoprotein A1 antibody [513]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:2000. IHC-P: 1:10-1:100. ELISA: 1microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, RadioImmunoAssay
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID513
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID335
- Target nameAPOA1
- Target descriptionapolipoprotein A1
- Target synonymsapo(a); apo-AI; apolipoprotein A-I; epididymis secretory sperm binding protein; HPALP2
- HostMouse
- IsotypeIgG1
- Protein IDP02647
- Protein NameApolipoprotein A-I
- Scientific DescriptionThis gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The encoded preproprotein is proteolytically processed to generate the mature protein, which promotes cholesterol efflux from tissues to the liver for excretion, and is a cofactor for lecithin cholesterolacyltransferase (LCAT), an enzyme responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein. [provided by RefSeq, Dec 2015]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203