Apolipoprotein B antibody
GTX27616
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Other Application
Product group Antibodies
TargetAPOB
Overview
- SupplierGeneTex
- Product NameApolipoprotein B antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:200-1:1000. IHC-P: 1:50-1:500. IP: 1:100. ELISA: 1:2000-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Other Application
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID338
- Target nameAPOB
- Target descriptionapolipoprotein B
- Target synonymsFCHL2, FLDB, LDLCQ4, apoB-100, apoB-48, apolipoprotein B-100, apolipoprotein B (including Ag(x) antigen), apolipoprotein B48
- HostGoat
- IsotypeIgG
- Protein IDP04114
- Protein NameApolipoprotein B-100
- Scientific DescriptionAt least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL and VLDL particles in human serum are often used to estimate an individuals relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins (especially A-1 and B) have been suggested to be more accurate estimators of coronary heart disease than measurements of lipoprotein particles (especially HDL and LDL). The apolipoproteins are derived from human plasma by density gradient ultracentrifugation and HPLC.
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Lipid-based strategies used to identify extracellular vesicles in flow cytometry can be confounded by lipoproteins: Evaluations of annexin V, lactadherin, and detergent lysis. Botha J et al., 2022 Apr, J Extracell VesiclesRead more
![ICC/IF analysis of HepG2 cells using GTX15663 Apolipoprotein B antibody [F2C13]. Panel e is a no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.1% Trito X-100 for 10 minutes Dilution : 2 microg/ml in 0.1% BSA and incubated for 3 hours at room temperature](https://www.genetex.com/upload/website/prouct_img/normal/GTX15663/GTX15663_276_ICC-IF_w_23060620_518.webp)
![IHC-P analysis of human liver tissue using GTX15664 Apolipoprotein B antibody [F2C4]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:20](https://www.genetex.com/upload/website/prouct_img/normal/GTX15664/GTX15664_1030_IHC-P_w_23060620_624.webp)
![IHC-P analysis of human liver tissue using GTX15665 Apolipoprotein B antibody [F2C3]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:20](https://www.genetex.com/upload/website/prouct_img/normal/GTX15665/GTX15665_1031_IHC-P_w_23060620_595.webp)
![ELISA analysis of antigen using GTX60445 Apolipoprotein B antibody [6G6]. Red : Control antigen 100ng Purple : Antigen 10ng Green : Antigen 50ng Blue : Antigen 100ng](https://www.genetex.com/upload/website/prouct_img/normal/GTX60445/GTX60445_20170912_ELISA_w_23061123_653.webp)