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Lane 1: Mouse placenta lysates probed with Anti –APPBP1 Polyclonal Antibody, Unconjugated (bs-12503R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:5000 for 90 min at 37˚C.
Lane 1: Mouse placenta lysates probed with Anti –APPBP1 Polyclonal Antibody, Unconjugated (bs-12503R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:5000 for 90 min at 37˚C.
Lane 1: Mouse placenta lysates probed with Anti –APPBP1 Polyclonal Antibody, Unconjugated (bs-12503R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:5000 for 90 min at 37˚C.

APPBP1 Polyclonal Antibody

BS-12503R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetNAE1
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Overview

  • Supplier
    Bioss
  • Product Name
    APPBP1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID8883
  • Target name
    NAE1
  • Target description
    NEDD8 activating enzyme E1 subunit 1
  • Target synonyms
    A-116A10.1, APPBP1, HPP1, NEDFIH, ula-1, NEDD8-activating enzyme E1 regulatory subunit, APP-BP1, NEDD8-activating enzyme E1 subunit, amyloid beta precursor protein binding protein 1, 59kDa, amyloid beta precursor protein-binding protein 1, 59 kDa, amyloid beta precursor protein-binding protein 1, 59kD, amyloid protein-binding protein 1, proto-oncogene protein 1, protooncogene protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13564
  • Protein Name
    NEDD8-activating enzyme E1 regulatory subunit
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of APPBP1/NAE1 using anti-APPBP1/NAE1 antibody (PB9339). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates, Lane 7: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APPBP1/NAE1 antigen affinity purified polyclonal antibody (Catalog # PB9339) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APPBP1/NAE1 at approximately 60 kDa. The expected band size for APPBP1/NAE1 is at 60 kDa.
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