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NAE1 / APPBP1 Antibody

LS-C404539
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetNAE1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    NAE1 / APPBP1 Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA, WB (1:200 - 1:1000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID8883
  • Target name
    NAE1
  • Target description
    NEDD8 activating enzyme E1 subunit 1
  • Target synonyms
    A-116A10.1, APPBP1, HPP1, NEDFIH, ula-1, NEDD8-activating enzyme E1 regulatory subunit, APP-BP1, NEDD8-activating enzyme E1 subunit, amyloid beta precursor protein binding protein 1, 59kDa, amyloid beta precursor protein-binding protein 1, 59 kDa, amyloid beta precursor protein-binding protein 1, 59kD, amyloid protein-binding protein 1, proto-oncogene protein 1, protooncogene protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of APPBP1/NAE1 using anti-APPBP1/NAE1 antibody (PB9339). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates, Lane 7: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APPBP1/NAE1 antigen affinity purified polyclonal antibody (Catalog # PB9339) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APPBP1/NAE1 at approximately 60 kDa. The expected band size for APPBP1/NAE1 is at 60 kDa.
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