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ProductsBack/Aquaporin 0 antibody
WB analysis of various sample lysates using GTX54297 Aquaporin 0 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX54297 Aquaporin 0 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX54297 Aquaporin 0 antibody. Dilution : 1:1000 Loading : 25microg per lane

Aquaporin 0 antibody

GTX54297
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetMIP
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Overview

  • Supplier
    GeneTex
  • Product Name
    Aquaporin 0 antibody
  • Delivery Days Customer
    7
  • Application Supplier Note
    WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4284
  • Target name
    MIP
  • Target description
    major intrinsic protein of lens fiber
  • Target synonyms
    AQP0; aquaporin 0; CTRCT15; lens fiber major intrinsic protein; LIM1; MIP26; MP26
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP30301
  • Protein Name
    Lens fiber major intrinsic protein
  • Scientific Description
    Major intrinsic protein is a member of the water-transporting aquaporins as well as the original member of the MIP family of channel proteins. The function of the fiber cell membrane protein encoded by this gene is undetermined, yet this protein is speculated to play a role in intracellular communication. The MIP protein is expressed in the ocular lens and is required for correct lens function. This gene has been mapped among aquaporins AQP2, AQP5, and AQP6, in a potential gene cluster at 12q13. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Aquaporin 0 using anti-Aquaporin 0 antibody (PB9811). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: rat eye ball tissue Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aquaporin 0 antigen affinity purified polyclonal antibody (Catalog # PB9811) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aquaporin 0 at approximately 28 kDa. The expected band size for Aquaporin 0 is at 28 kDa.
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