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Western Blot analysis of HT29 cells using AQP0 Polyclonal Antibody
Western Blot analysis of HT29 cells using AQP0 Polyclonal Antibody
Western Blot analysis of HT29 cells using AQP0 Polyclonal Antibody

MIP Antibody

CSB-PA000910
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMIP
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Overview

  • Supplier
    Cusabio
  • Product Name
    MIP Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4284
  • Target name
    MIP
  • Target description
    major intrinsic protein of lens fiber
  • Target synonyms
    AQP0, CTRCT15, LIM1, MIP26, MP26, lens fiber major intrinsic protein, aquaporin 0
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP30301
  • Protein Name
    Lens fiber major intrinsic protein
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Aquaporin 0 using anti-Aquaporin 0 antibody (PB9811). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: rat eye ball tissue Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aquaporin 0 antigen affinity purified polyclonal antibody (Catalog # PB9811) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aquaporin 0 at approximately 28 kDa. The expected band size for Aquaporin 0 is at 28 kDa.
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