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ASAH1 Polyclonal Antibody

RD77849A
Reddot Biotech
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetASAH1
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Overview

  • Supplier
    Reddot Biotech
  • Product Name
    ASAH1 Polyclonal Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.3 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID427
  • Target name
    ASAH1
  • Target description
    N-acylsphingosine amidohydrolase 1
  • Target synonyms
    AC; ACDase; acid CDase; acid ceramidase; acylsphingosine deacylase; ASAH; N-acylethanolamine hydrolase ASAH1; N-acylsphingosine amidohydrolase (acid ceramidase) 1; PHP; PHP32; putative 32 kDa heart protein; SMAPME
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    This gene encodes a heterodimeric protein consisting of a nonglycosylated alpha subunit and a glycosylated beta subunit that is cleaved to the mature enzyme posttranslationally. The encoded protein catalyzes the synthesis and degradation of ceramide into sphingosine and fatty acid. Mutations in this gene have been associated with a lysosomal storage disorder known as Farber disease. Multiple transcript variants encoding several distinct isoforms have been identified for this gene.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ASAH1 using anti-ASAH1 antibody (A02055-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat heart tissue lysates, Lane 3: mouse heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ASAH1 antigen affinity purified polyclonal antibody (Catalog # A02055-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ASAH1 at approximately 45KD. The expected band size for ASAH1 is at 45KD.
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