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ASCL1 antibody

70R-49316
Biosynth
Product group Antibodies
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Overview

  • Supplier
    Biosynth
  • Product Name
    ASCL1 antibody
  • Delivery Days Customer
    11
  • Certification
    Research Use Only
  • UNSPSC
    12352203

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A549 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ASCL1 Antibody(bs-1155R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Product group Antibodies
References
Bioss
ASCL1 Polyclonal AntibodyBS-1155R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetASCL1
  • SizePrice
Product group Antibodies
Biomatik
ASCL1 Polyclonal AntibodyCAC15005
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetASCL1
  • SizePrice
Figure 1. Western blot analysis of ASCL1 using anti-ASCL1 antibody (A03023-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human NCL-H460 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ASCL1 antigen affinity purified polyclonal antibody (Catalog # A03023-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ASCL1 at approximately 30 kDa. The expected band size for ASCL1 is at 25 kDa.
Product group Antibodies
Boster Bio
Anti-ASCL1 Antibody Picoband(r)A03023-2-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
TargetASCL1
  • SizePrice
Product group Antibodies
RayBiotech
Anti-ASCL1 (Achaete-scute homolog 1) (C-term D220) Antibody102-23801
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TargetASCL1
  • SizePrice
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Product group Antibodies
Antibodies.com
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Product group Antibodies
Absolute Antibody
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Product group Antibodies
References
GeneTex
ASCL1 antibodyGTX129189
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
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LifeSpan BioSciences
ASCL1 / MASH1 AntibodyLS-C402152
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Product group Antibodies
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