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Western Blot Positive WB detected in: Rat brain tissue All lanes: ASCL1 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 26 kDa Observed band size: 26 kDa
Western Blot Positive WB detected in: Rat brain tissue All lanes: ASCL1 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 26 kDa Observed band size: 26 kDa
Western Blot Positive WB detected in: Rat brain tissue All lanes: ASCL1 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 26 kDa Observed band size: 26 kDa

ASCL1 Antibody

CSB-PA002199LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Rat
TargetASCL1
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Overview

  • Supplier
    Cusabio
  • Product Name
    ASCL1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID429
  • Target name
    ASCL1
  • Target description
    achaete-scute family bHLH transcription factor 1
  • Target synonyms
    ASH1, HASH1, MASH1, bHLHa46, achaete-scute homolog 1, ASH-1, achaete scute protein, achaete-scute complex homolog 1, achaete-scute complex-like 1, class A basic helix-loop-helix protein 46
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP50553
  • Protein Name
    Achaete-scute homolog 1
  • Scientific Description
    Transcription factor that controls transcriptional expression of its target genes by binding to the E box (5-CANNTG-3). Plays a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. Acts synergistically with FOXN4 to specify the identity of V2b neurons rather than V2a from bipotential p2 progenitors during spinal cord neurogenesis, probably through DLL4-NOTCH signaling activation. Essential for the generation of olfactory and autonomic neurons (By similarity).
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ASCL1 using anti-ASCL1 antibody (A03023-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human NCL-H460 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ASCL1 antigen affinity purified polyclonal antibody (Catalog # A03023-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ASCL1 at approximately 30 kDa. The expected band size for ASCL1 is at 25 kDa.
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