
Formalin-fixed and paraffin embedded mouse embryo labeled with Anti ATAD5 Polyclonal Antibody,Unconjugated (bs-7656R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
ATAD5 Polyclonal Antibody
BS-7656R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetATAD5
Overview
- SupplierBioss
- Product NameATAD5 Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID79915
- Target nameATAD5
- Target descriptionATPase family AAA domain containing 5
- Target synonymsC17orf41, ELG1, FRAG1, ATPase family AAA domain-containing protein 5, chromosome fragility associated gene 1, chromosome fragility-associated gene 1 protein, enhanced level of genomic instability 1 homolog
- HostRabbit
- IsotypeIgG
- Protein IDQ96QE3
- Protein NameATPase family AAA domain-containing protein 5
- Storage Instruction-20°C
- UNSPSC12352203


![Western blot using GeneTex Affinity Purified anti-Elg1 antibody (GTX25429) shows detection of a band ~120 kDa corresponding to human Elg1 in various cell lysates. Lanes contain ~ 5ug of HeLa nuclear extract (1), HeLa (2), A431 (3), Jurkat (4) and HEK293 (5) whole cell lysates. After SDS-PAGE, transfer and blocking, the membrane was probed with the primary antibody diluted to 1:500. The membrane was then washed and reacted with a HRP conjugated Gt-a-Rabbit IgG [H&L] MX followed by ECL detection using a 2 m exposure time. The expected molecular weight of Elg1 is 120kDa according to Kanellis P et al. 2003, although the predicted molecular weight is 207 kDa. The 50kD bands in Jurkat and 293 cell lysates are probably cross-reaction with other proteins. Both the 120 kDa and 50 kDa bands are not observed when antibody is pre-incubated with peptide (data not shown](https://www.genetex.com/upload/website/prouct_img/normal/GTX25429/GTX25429_20160330_WB_w_23060722_890.webp)


