Untreated (-) and treated (+) NIH-3T3 whole cell extracts (30 microg) were separated by 10% SDS-PAGE, and the membrane was blotted with ATF2 (phospho Thr71) antibody [GT1275] (GTX03187) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![WB analysis of NIH-3T3 cells were treated by Anisomycin (25 ug/mL) at 37oC for 30 minutes using GTX03187 ATF2 (phospho Thr71) antibody [GT1275]. Dilution : 1:1000 Loading : 25microg per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX03187/GTX03187_94_WB_w_23053123_312.webp "WB analysis of NIH-3T3 cells were treated by Anisomycin (25 ug/mL) at 37oC for 30 minutes using GTX03187 ATF2 (phospho Thr71) antibody [GT1275]. Dilution : 1:1000 Loading : 25microg per lane")
Untreated (-) and treated (+) NIH-3T3 whole cell extracts (30 microg) were separated by 10% SDS-PAGE, and the membrane was blotted with ATF2 (phospho Thr71) antibody [GT1275] (GTX03187) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
ATF2 (phospho Thr71) antibody [GT1275]
GTX03187
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetATF2
Overview
- SupplierGeneTex
- Product NameATF2 (phospho Thr71) antibody [GT1275] - Orthogonal Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500 - 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT1275
- Concentration0.52 mg/ml
- ConjugateUnconjugated
- FormulationLiquid
- Gene ID1386
- Target nameATF2
- Target descriptionactivating transcription factor 2
- Target synonymsactivating transcription factor 2 splice variant ATF2-var2; cAMP response element-binding protein CRE-BP1; cAMP responsive element binding protein 2, formerly; cAMP-dependent transcription factor ATF-2; CREB2; CREB-2; CRE-BP1; cyclic AMP-dependent transcription factor ATF-2; cyclic AMP-responsive element-binding protein 2; HB16; histone acetyltransferase ATF2; TREB7
- HostRabbit
- IsotypeIgG
- Modification TypePhosphorylated
- Protein IDP15336
- Protein NameCyclic AMP-dependent transcription factor ATF-2
- Scientific DescriptionThis gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. This protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. The encoded protein may also be involved in cells DNA damage response independent of its role in transcriptional regulation. Several alternatively spliced transcript variants have been found for this gene [provided by RefSeq, Jan 2014]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203