WB analysis of various sample lysates using GTX30069 ATF3 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX30069 ATF3 antibody. Dilution : 1:1000 Loading : 25microg per lane
ATF3 antibody
GTX30069
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetATF3
Overview
- SupplierGeneTex
- Product NameATF3 antibody
- Delivery Days Customer7
- Application Supplier NoteWB: 1:500 - 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- Gene ID467
- Target nameATF3
- Target descriptionactivating transcription factor 3
- Target synonymscyclic AMP-dependent transcription factor ATF-3, cAMP-dependent transcription factor ATF-3
- HostRabbit
- IsotypeIgG
- Protein IDP18847
- Protein NameCyclic AMP-dependent transcription factor ATF-3
- Scientific DescriptionThis gene encodes a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. This gene is induced by a variety of signals, including many of those encountered by cancer cells, and is involved in the complex process of cellular stress response. Multiple transcript variants encoding different isoforms have been found for this gene. It is possible that alternative splicing of this gene may be physiologically important in the regulation of target genes. [provided by RefSeq, Apr 2011]
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
Datasheet
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![Western blot of E.coli whole cell extract transfected with GST epitope tagged human ATF3. GeneTexs Affinity purified anti-ATF3 detects a band ~48 kDa corresponding to recombinant human ATF3. Immunostaining using GeneTexs anti-GST epitope tag antibody confirms the composition of the recombinant band (not shown). The protein was transferred to nitrocellulose using standard methods. After blocking with 5% goat serum and 0.5% non fat milk in PBS, the membrane was probed with the primary antibody diluted 1:200 in 0.2X blocking buffer in PBS overnight at 4oC. Reaction was followed by washes and reaction with a 1:5000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] for 30 min at room temperature. LICORs OdysseyR Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.](https://www.genetex.com/upload/website/prouct_img/normal/GTX48669/GTX48669_20160330_WB_1_w_23060823_956.webp)
Product group Antibodies
References
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