Western blot of E.coli whole cell extract transfected with GST epitope tagged human ATF3. GeneTexs Affinity purified anti-ATF3 detects a band ~48 kDa corresponding to recombinant human ATF3. Immunostaining using GeneTexs anti-GST epitope tag antibody confirms the composition of the recombinant band (not shown). The protein was transferred to nitrocellulose using standard methods. After blocking with 5% goat serum and 0.5% non fat milk in PBS, the membrane was probed with the primary antibody diluted 1:200 in 0.2X blocking buffer in PBS overnight at 4oC. Reaction was followed by washes and reaction with a 1:5000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] for 30 min at room temperature. LICORs OdysseyR Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
![Western blot of mammalian whole cell extract transfected with HA epitope tagged human ATF3. GeneTexs Affinity purified anti-ATF3 detects a band ~31 kDa corresponding to recombinant human ATF3. Immunostaining using GeneTexs anti-HA epitope tag antibody confirms the composition of the recombinant band (not shown). The protein was transferred to nitrocellulose in 30 minutes using standard methods. After blocking with 5% goat serum and 0.5% non-fat milk in PBS, the membrane was probed with the primary antibody diluted 1:200 in 0.2X blocking buffer in PBS overnight at 4oC. Reaction was followed by washes and reaction with a 1:5000 dilution of IRDye?800 conjugated Gt-a-Rabbit IgG [H&L] for 30 min at room temperature. LICORs OdysseyR Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.](https://www.genetex.com/upload/website/prouct_img/normal/GTX48669/GTX48669_20160330_WB_w_23060823_381.webp "Western blot of mammalian whole cell extract transfected with HA epitope tagged human ATF3. GeneTexs Affinity purified anti-ATF3 detects a band ~31 kDa corresponding to recombinant human ATF3. Immunostaining using GeneTexs anti-HA epitope tag antibody confirms the composition of the recombinant band (not shown). The protein was transferred to nitrocellulose in 30 minutes using standard methods. After blocking with 5% goat serum and 0.5% non-fat milk in PBS, the membrane was probed with the primary antibody diluted 1:200 in 0.2X blocking buffer in PBS overnight at 4oC. Reaction was followed by washes and reaction with a 1:5000 dilution of IRDye?800 conjugated Gt-a-Rabbit IgG [H&L] for 30 min at room temperature. LICORs OdysseyR Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.")
Western blot of E.coli whole cell extract transfected with GST epitope tagged human ATF3. GeneTexs Affinity purified anti-ATF3 detects a band ~48 kDa corresponding to recombinant human ATF3. Immunostaining using GeneTexs anti-GST epitope tag antibody confirms the composition of the recombinant band (not shown). The protein was transferred to nitrocellulose using standard methods. After blocking with 5% goat serum and 0.5% non fat milk in PBS, the membrane was probed with the primary antibody diluted 1:200 in 0.2X blocking buffer in PBS overnight at 4oC. Reaction was followed by washes and reaction with a 1:5000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] for 30 min at room temperature. LICORs OdysseyR Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
ATF3 antibody
GTX48669
Overview
- SupplierGeneTex
- Product NameATF3 antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:200-1:2000. ELISA: 1:5000-1:20000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID467
- Target nameATF3
- Target descriptionactivating transcription factor 3
- Target synonymscAMP-dependent transcription factor ATF-3; cyclic AMP-dependent transcription factor ATF-3
- HostRabbit
- IsotypeIgG
- Protein IDP18847
- Protein NameCyclic AMP-dependent transcription factor ATF-3
- Scientific DescriptionATF3, or Activating Transcription Factor 3, is a member of mammalian activation TF/CREB protein family of transcription factors. ATF3 binds the cAMP response element (cre) (consensus: 5-gtgacgt[ac][ag]-3), a sequence present in many viral and cellular promoters. However, ATF3 represses rather than activates transcription from promoters with ATF sites stabilizing inhibitory co-factors at the promoter. Alternate splicing forms of ATF3, called ATF3 delta Zip, lack the leucine zipper domain and do not bind DNA. ATF3 delta Zip stimulates transcription, presumably by sequestering inhibitory co-factors away from the promoter. Human ATF3 (SwissProt 18847) is a 20575 Da protein composed of 181 amino acids.
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203