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Western Blot analysis of Jurkat cells using ATF-5 Polyclonal Antibody
Western Blot analysis of Jurkat cells using ATF-5 Polyclonal Antibody
Western Blot analysis of Jurkat cells using ATF-5 Polyclonal Antibody

ATF5 Antibody

CSB-PA000948
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetATF5
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Overview

  • Supplier
    Cusabio
  • Product Name
    ATF5 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID22809
  • Target name
    ATF5
  • Target description
    activating transcription factor 5
  • Target synonyms
    ATFX; cAMP-dependent transcription factor ATF-5; cyclic AMP-dependent transcription factor ATF-5; HMFN0395; transcription factor ATFx
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y2D1
  • Protein Name
    Cyclic AMP-dependent transcription factor ATF-5
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ATF5 using anti-ATF5 antibody (A02792-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATF5 antigen affinity purified polyclonal antibody (Catalog # A02792-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATF5 at approximately 37 kDa. The expected band size for ATF5 is at 31 kDa.
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