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WB analysis of Jurkat cell lysate using GTX34045 ATF5 antibody.
WB analysis of Jurkat cell lysate using GTX34045 ATF5 antibody.
WB analysis of Jurkat cell lysate using GTX34045 ATF5 antibody.

ATF5 antibody

GTX34045
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetATF5
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Overview

  • Supplier
    GeneTex
  • Product Name
    ATF5 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID22809
  • Target name
    ATF5
  • Target description
    activating transcription factor 5
  • Target synonyms
    ATFX; cAMP-dependent transcription factor ATF-5; cyclic AMP-dependent transcription factor ATF-5; HMFN0395; transcription factor ATFx
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y2D1
  • Protein Name
    Cyclic AMP-dependent transcription factor ATF-5
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ATF5 using anti-ATF5 antibody (A02792-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATF5 antigen affinity purified polyclonal antibody (Catalog # A02792-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATF5 at approximately 37 kDa. The expected band size for ATF5 is at 31 kDa.
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