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IHC-P analysis of mouse kidney tissue using GTX15457 AFT6 antibody. Antigen retrieval : Heat mediation with citrate buffer(pH6). Dilution : 5microg/ml
IHC-P analysis of mouse kidney tissue using GTX15457 AFT6 antibody. Antigen retrieval : Heat mediation with citrate buffer(pH6). Dilution : 5microg/ml
IHC-P analysis of mouse kidney tissue using GTX15457 AFT6 antibody. Antigen retrieval : Heat mediation with citrate buffer(pH6). Dilution : 5microg/ml

ATF6 antibody

GTX15457
GeneTex
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetATF6
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Overview

  • Supplier
    GeneTex
  • Product Name
    ATF6 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.5-2 microg/mL. IHC-P: 5 microg/mL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID22926
  • Target name
    ATF6
  • Target description
    activating transcription factor 6
  • Target synonyms
    ACHM7, ATF6A, ATP6alpha, cyclic AMP-dependent transcription factor ATF-6 alpha, cAMP-dependent transcription factor ATF-6 alpha
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP18850
  • Protein Name
    Cyclic AMP-dependent transcription factor ATF-6 alpha
  • Scientific Description
    ATF6 is an endoplasmic reticulum (ER) stress-regulated transmembrane transcription factor that activates the transcription of ER molecules.[supplied by OMIM]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Ge L, Wang T, Shi D, et al. ATF6α contributes to rheumatoid arthritis by inducing inflammatory cytokine production and apoptosis resistance. Front Immunol. 2022,13:965708. doi: 10.3389/fimmu.2022.965708
    Read this paper

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Figure 1. Western blot analysis of ATF6 using anti-ATF6 antibody (A00655-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATF6 antigen affinity purified polyclonal antibody (Catalog # A00655-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATF6 at approximately 90-100 kDa. The expected band size for ATF6 is at 75 kDa.
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WB analysis of NIH-3T3 cell lysate using GTX00686 ATF6 antibody [37-1]. The asterisk denotes an unglycosylated form of pATF6alpha(P). ATF6alpha is constitutively expressed as pATF6alpha(P), and converted to pATF6alpha(N) ( > 50-kDa protein) in ER-stressed cells. pATF6alpha(P) : the endogenous precursor ATF6alpha (~90kDa) pATF6alpha(N) : the cleaved product of ATF6alpha(>50kDa) Lane 1 : untreated Lane 2 : 1mM DTT (reducing reagent) for 1 h Lane 3 : 2 microg/ml tunicamycin (inhibitor of N-glycosylation) for 3 h Lane 4 : 2 microg/ml tunicamycin (inhibitor of N-glycosylation) for 7 h. Loading : 50microg
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ICC/IF analysis of HeLa cells using GTX00687 ATF6 antibody [1-7]. Green : Primary antibody Blue : DAPI Dilution : 1:100
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WB analysis of HeLa whole cell lysate using GTX81096 ATF6 antibody, Internal. Loading : 20 microg per lane Dilution : 1:16000
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