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Western Blot analysis of 293T cell using ATG9A Polyclonal Antibody at dilution of 1:240
Western Blot analysis of 293T cell using ATG9A Polyclonal Antibody at dilution of 1:240
Western Blot analysis of 293T cell using ATG9A Polyclonal Antibody at dilution of 1:240

ATG9A Polyclonal Antibody

E-AB-14724
Elabscience
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetATG9A
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Overview

  • Supplier
    Elabscience
  • Product Name
    ATG9A Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    ELISA WB IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.2 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID79065
  • Target name
    ATG9A
  • Target description
    autophagy related 9A
  • Target synonyms
    APG9L1, MGD3208, mATG9, autophagy-related protein 9A, APG9 autophagy 9-like 1, APG9-like 1, ATG9 autophagy related 9 homolog A, autophagy 9-like 1 protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ7Z3C6
  • Protein Name
    Autophagy-related protein 9A
  • Scientific Description
    Autophagy-related protein 9Ais aproteinthat in humans is encoded by theATG9Agene. Involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS), the nucleating site for formation of the sequestering vesicle. Cycles between a juxta-nuclear trans-Golgi network compartment and late endosomes. Nutrient starvation induces accumulation on autophagosomes.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ATG9A using anti-ATG9A antibody (A03757-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human A375 whole cell lysates, Lane 4: human Mcf-7 whole cell lysates, Lane 5: human K562 whole cell lysates, Lane 6: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATG9A antigen affinity purified polyclonal antibody (Catalog # A03757-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATG9A at approximately 94KD. The expected band size for ATG9A is at 94KD.
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