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ProductsBack/ATGL (F-7) Alexa Fluor(r) 790

ATGL (F-7) Alexa Fluor(r) 790

SC-365278AF790
Santa Cruz Biotechnology
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPNPLA2
Price on request
200 ug
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Overview

  • Supplier
    Santa Cruz Biotechnology
  • Product Name
    ATGL (F-7) Alexa Fluor(r) 790
  • Delivery Days Customer
    5
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Conjugate
    AlexaFluor 790
  • Gene ID57104
  • Target name
    PNPLA2
  • Target description
    patatin like domain 2, triacylglycerol lipase
  • Target synonyms
    1110001C14Rik, ATGL, FP17548, PEDF-R, TTS-2.2, TTS2, iPLA2zeta, patatin-like phospholipase domain-containing protein 2, IPLA2-zeta, TTS2.2, adipose triglyceride lipase, calcium-independent phospholipase A2, calcium-independent phospholipase A2-zeta, desnutrin, mutant patatin-like phospholipase domain containing 2, patatin like phospholipase domain containing 2, pigment epithelium-derived factor, pigment epithelium-derived factor receptor, transport-secretion protein 2.2, triglyceride hydrolase
  • Host
    Mouse
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ATGL/PNPLA2 using anti-ATGL/PNPLA2 antibody (M01800). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human SiHa whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: rat heart tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates, Lane 8: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATGL/PNPLA2 antigen affinity purified monoclonal antibody (Catalog # M01800) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATGL/PNPLA2 at approximately 55 kDa. The expected band size for ATGL/PNPLA2 is at 55 kDa.
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