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Atic Polyclonal Antibody

CAC11757
Biomatik
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetATIC
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Overview

  • Supplier
    Biomatik
  • Product Name
    ATIC Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, IHC, IF; Recommended dilution: IHC:1:20-1:200, IF:1:50-1:200
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID471
  • Target name
    ATIC
  • Target description
    5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase
  • Target synonyms
    5-aminoimidazole-4-carboxamide-1-beta-D-ribonucleotide transformylase/inosinicase; AICAR; AICAR formyltransferase/IMP cyclohydrolase bifunctional enzyme; AICAR transformylase/inosine monophosphate cyclohydrolase; AICARFT; AICARFT/IMPCHASE; bifunctional purine biosynthesis protein ATIC; bifunctional purine biosynthesis protein PURH; epididymis secretory sperm binding protein Li 70p; HEL-S-70p; IMPCHASE; phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase; PURH
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP31939
  • Protein Name
    Bifunctional purine biosynthesis protein PURH
  • Scientific Description
    The Atic Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, IHC, IF.
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ATIC using anti-ATIC antibody (A05404-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human HEK293 whole cell lysates, Lane 5: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATIC antigen affinity purified polyclonal antibody (Catalog # A05404-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATIC at approximately 65 kDa. The expected band size for ATIC is at 65 kDa.
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