Bio-Connect
Lane 1: K562 cell lysates; Lane 2: HepG2 cell lysates probed with ATIC Polyclonal Antibody, Unconjugated (bs-12544R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: K562 cell lysates; Lane 2: HepG2 cell lysates probed with ATIC Polyclonal Antibody, Unconjugated (bs-12544R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: K562 cell lysates; Lane 2: HepG2 cell lysates probed with ATIC Polyclonal Antibody, Unconjugated (bs-12544R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

ATIC/PURH Polyclonal Antibody

BS-12544R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetATIC
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    ATIC/AICAR transformylase Antibody
  • Delivery Days Customer
    7
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID471
  • Target name
    ATIC
  • Target description
    5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase
  • Target synonyms
    5-aminoimidazole-4-carboxamide-1-beta-D-ribonucleotide transformylase/inosinicase; AICAR; AICAR formyltransferase/IMP cyclohydrolase bifunctional enzyme; AICAR transformylase/inosine monophosphate cyclohydrolase; AICARFT; AICARFT/IMPCHASE; bifunctional purine biosynthesis protein ATIC; bifunctional purine biosynthesis protein PURH; epididymis secretory sperm binding protein Li 70p; HEL-S-70p; IMPCHASE; phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase; PURH
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Related products

Product group Antibodies
Biomatik
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
TargetATIC
  • SizePrice
Product group Antibodies
Assay Genie
TargetATIC
  • SizePrice
Figure 1. Western blot analysis of ATIC using anti-ATIC antibody (A05404-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human HEK293 whole cell lysates, Lane 5: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATIC antigen affinity purified polyclonal antibody (Catalog # A05404-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATIC at approximately 65 kDa. The expected band size for ATIC is at 65 kDa.
Product group Antibodies
Boster Bio
Anti-ATIC Antibody Picoband(r)A05404-2-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA
TargetATIC
  • SizePrice
WB analysis of various sample lysates using GTX33026 ATIC antibody. Dilution : 1:1000 Loading : 25microg per lane
Product group Antibodies
GeneTex
ApplicationsWestern Blot
TargetATIC
  • SizePrice
Product group Antibodies
MedChem Express
TargetATIC
  • SizePrice
Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.
Product group Antibodies
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetATIC
  • SizePrice
Immunohistochemistry of paraffin-embedded human lymph node tissue using CSB-PA002299LA01HU at dilution of 1:100
Product group Antibodies
Cusabio
ATIC AntibodyCSB-PA002299LA01HU
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
TargetATIC
  • SizePrice
Product group Antibodies
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot
TargetATIC
  • SizePrice
Product group Antibodies
RayBiotech
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetATIC
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
TargetATIC
  • SizePrice