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Anti-ATIC Antibody

CAB5559
Assay Genie
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetATIC
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Overview

  • Supplier
    Assay Genie
  • Product Name
    Anti-ATIC Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    Not applicable
  • Conjugate
    Unconjugated
  • Gene ID471
  • Target name
    ATIC
  • Target description
    5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase
  • Target synonyms
    AICAR, AICARFT, HEL-S-70p, IMPCHASE, PURH, bifunctional purine biosynthesis protein ATIC, 5-aminoimidazole-4-carboxamide-1-beta-D-ribonucleotide transformylase/inosinicase, AICAR formyltransferase/IMP cyclohydrolase bifunctional enzyme, AICAR transformylase/inosine monophosphate cyclohydrolase, AICARFT/IMPCHASE, bifunctional purine biosynthesis protein PURH, epididymis secretory sperm binding protein Li 70p, phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase
  • Host
    Rabbit
  • Protein IDP31939
  • Protein Name
    Bifunctional purine biosynthesis protein ATIC
  • Scientific Description
    ATIC Antibody is a premium polyclonal antibody that offers outstanding performance and reliability for demanding research applications. Rigorously validated for WB, ELISA, this antibody ensures consistent, reproducible results across multiple experimental platforms. Demonstrates excellent reactivity with Human samples, providing researchers with confidence in cross-species compatibility. Conveniently packaged in 100microL format to meet your experimental needs. For optimal performance, store at -20°C and maintains stability for 12 months. Backed by rigorous quality control testing to ensure superior performance in your critical research applications.
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity
    Human
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of ATIC using anti-ATIC antibody (A05404-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human HEK293 whole cell lysates, Lane 5: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATIC antigen affinity purified polyclonal antibody (Catalog # A05404-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATIC at approximately 65 kDa. The expected band size for ATIC is at 65 kDa.
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