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ATIC Antibody (YA1072)

HY-P81327
MedChem Express
TargetATIC
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    ATIC Antibody (YA1072)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID471
  • Target name
    ATIC
  • Target description
    5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase
  • Target synonyms
    5-aminoimidazole-4-carboxamide-1-beta-D-ribonucleotide transformylase/inosinicase; AICAR; AICAR formyltransferase/IMP cyclohydrolase bifunctional enzyme; AICAR transformylase/inosine monophosphate cyclohydrolase; AICARFT; AICARFT/IMPCHASE; bifunctional purine biosynthesis protein ATIC; bifunctional purine biosynthesis protein PURH; epididymis secretory sperm binding protein Li 70p; HEL-S-70p; IMPCHASE; phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase; PURH
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    ATIC Antibody (YA1072) is a non-conjugated IgG1 antibody, targeting ATIC, with a predicted molecular weight of 65 kDa (observed band size: 65 kDa). ATIC Antibody (YA1072) can be used for WB experiment in human, mouse, rat background.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ATIC using anti-ATIC antibody (A05404-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human HEK293 whole cell lysates, Lane 5: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATIC antigen affinity purified polyclonal antibody (Catalog # A05404-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATIC at approximately 65 kDa. The expected band size for ATIC is at 65 kDa.
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