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ProductsBack/ATIC Mouse Monoclonal Antibody [Clone ID: F38 P7 H9]
Figure 2. Western blot using ATIC antibody on HT29 cell lysate.
Figure 2. Western blot using ATIC antibody on HT29 cell lysate.
Figure 2. Western blot using ATIC antibody on HT29 cell lysate.

ATIC Mouse Monoclonal Antibody [Clone ID: F38 P7 H9]

AM05383PU-N
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityAmphibian, Drosophila, Human, Mouse, Rat
TargetATIC
Price on request
200 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    ATIC Mouse Monoclonal Antibody [Clone ID: F38 P7 H9]
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    F38 P7 H9
  • Gene ID471
  • Target name
    ATIC
  • Target description
    5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase
  • Target synonyms
    AICAR, AICARFT, HEL-S-70p, IMPCHASE, PURH, bifunctional purine biosynthesis protein ATIC, 5-aminoimidazole-4-carboxamide-1-beta-D-ribonucleotide transformylase/inosinicase, AICAR formyltransferase/IMP cyclohydrolase bifunctional enzyme, AICAR transformylase/inosine monophosphate cyclohydrolase, AICARFT/IMPCHASE, bifunctional purine biosynthesis protein PURH, epididymis secretory sperm binding protein Li 70p, phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP31939
  • Protein Name
    Bifunctional purine biosynthesis protein ATIC
  • Scientific Description
    ATIC mouse monoclonal antibody, clone F38 P7 H9, Purified
  • Reactivity
    Amphibian, Drosophila, Human, Mouse, Rat
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of ATIC using anti-ATIC antibody (A05404-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human HEK293 whole cell lysates, Lane 5: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATIC antigen affinity purified polyclonal antibody (Catalog # A05404-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATIC at approximately 65 kDa. The expected band size for ATIC is at 65 kDa.
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