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Western Blot analysis of A549 cells using Na+/K+-ATPase alpha1 Polyclonal Antibody
Western Blot analysis of A549 cells using Na+/K+-ATPase alpha1 Polyclonal Antibody
Western Blot analysis of A549 cells using Na+/K+-ATPase alpha1 Polyclonal Antibody

ATP1A1 Antibody

CSB-PA003364
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetATP1A1
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Overview

  • Supplier
    Cusabio
  • Product Name
    ATP1A1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID476
  • Target name
    ATP1A1
  • Target description
    ATPase Na+/K+ transporting subunit alpha 1
  • Target synonyms
    ATPase, Na+/K+ transporting, alpha 1 polypeptide; CMT2DD; HOMGSMR2; Na(+)/K(+) ATPase alpha-1 subunit; Na, K-ATPase, alpha-A catalytic polypeptide; Na,K-ATPase alpha-1 subunit; Na,K-ATPase catalytic subunit alpha-A protein; Na+/K+ ATPase 1; sodium pump subunit alpha-1; sodium/potassium-transporting ATPase subunit alpha-1; sodium-potassium ATPase catalytic subunit alpha-1; sodium-potassium-ATPase, alpha 1 polypeptide
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP05023
  • Protein Name
    Sodium/potassium-transporting ATPase subunit alpha-1
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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IHC-P analysis of human colon carcinoma tissue using GTX22872 Sodium/Potassium ATPase alpha 1 antibody [M8-P1-A3]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200
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Formalin-fixed and paraffin embedded human bladder carcinoma labeled with Anti-ATP1A1 Polyclonal Antibody, Unconjugated (bs-4255R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
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Figure 1. Western blot analysis of ATP1A1 using anti-ATP1A1 antibody (PB9482). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates, Lane 4: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP1A1 antigen affinity purified polyclonal antibody (Catalog # PB9482) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-DyLight 647 Conjugated secondary antibody (Left) at a dilution of 1:2000 or a goat anti-rabbit IgG-HRP Conjugated secondary antibody (Right) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATP1A1 approximately 100 kDa. The expected band size for ATP1A1 is at 74, 110, 113 kDa.
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