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ProductsBack/ATP2B1 / PMCA1 Antibody (clone 3E2)

ATP2B1 / PMCA1 Antibody (clone 3E2)

LS-C196715
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetATP2B1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ATP2B1 / PMCA1 Antibody (clone 3E2)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Sandwich ELISA: Recombinant protein. Western Blot: Recombinant protein.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA, WB Sandwich ELISA: Recombinant protein. Western Blot: Recombinant protein.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    3E2
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID490
  • Target name
    ATP2B1
  • Target description
    ATPase plasma membrane Ca2+ transporting 1
  • Target synonyms
    MRD66, PMCA1, PMCA1kb, plasma membrane calcium-transporting ATPase 1, ATPase, Ca++ transporting, plasma membrane 1, plasma membrane calcium pump
  • Host
    Mouse
  • Isotype
    IgG2a
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PMCA1 using anti-PMCA1 antibody (M02669). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PMCA1 antigen affinity purified monoclonal antibody (Catalog # M02669) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PMCA1 at approximately 250 kDa. The expected band size for PMCA1 is at 135 kDa.
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