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ProductsBack/Goat anti-ATP2B1 (aa312-327)

Goat anti-ATP2B1 (aa312-327)

EB11182
Everest Biotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetATP2B1
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-ATP2B1 (aa312-327) Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID490
  • Target name
    ATP2B1
  • Target description
    ATPase plasma membrane Ca2+ transporting 1
  • Target synonyms
    ATPase, Ca++ transporting, plasma membrane 1; plasma membrane calcium pump; plasma membrane calcium-transporting ATPase 1; PMCA1; PMCA1kb
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_001001323.1; NP_001673.2. GeneIDs all Nonhuman: 67972 (mouse); 29598 (rat);. Purification: Antigen affinity purified. Names and symbols: ATP2B1; ATPase, Ca++ transporting, plasma membrane 1; PMCA1; PMCA1kb; plasma membrane calcium ATPase; plasma membrane calcium pump; plasma membrane calcium-ATPase; plasma membrane calcium-transporting ATPase 1
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Pig, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PMCA1 using anti-PMCA1 antibody (M02669). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PMCA1 antigen affinity purified monoclonal antibody (Catalog # M02669) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PMCA1 at approximately 250 kDa. The expected band size for PMCA1 is at 135 kDa.
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